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Detection of abortifacient agents in domestic ruminants, with a specific focus on Coxiella burnetii

René van den Brom ,
Susan Neale ,
Elsa Jourdain
,
Anneleen Matthijs
,
Marcella Mori ,
Elodie Rousset
,
Katja Mertens-Scholz
,
Tom N. McNeilly ,
Ana Hurtado

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March 31, 2025

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https://doi.org/10.12688/openreseurope.19270.1

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June 30, 2025 (Version of Record 1.0)
March 31, 2025 (Published Manuscript Under Review 1.0)

European Commission

Grant ID(s): 862605

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ruminants, abortion, diagnostics, Coxiella burnetii

Abstract

Q fever is a widespread zoonotic disease caused by the bacterium, Coxiella burnetii. In ruminants, C. burnetii can cause abortions, stillbirths, premature births, and weak offspring. As part of the EU-funded Q-Net-Assess International Coordination of Research on Infectious Animal Diseases (ICRAD)-project, aimed at generating the most comprehensive understanding of C. burnetii genetic variation to date and determining the implications of this genetic variation for zoonotic risk, pathogenicity and control of C. burnetii infection, we have reviewed the protocols reported by the different project partners and/or countries to diagnose abortion in domestic ruminants. As a result of this review, we have developed guidelines for the detection of abortifacient agents in domestic ruminants, with a special focus on C. burnetii. They include a description of the essential and complementary samples needed for a definitive diagnosis, the analytical techniques to be used, and the interpretation and validity of each type of sample and technique. The most comprehensive diagnostic approach to identify an infectious agent as the cause of abortion in ruminants would include histopathology, including immunohistochemistry (IHC), on the fetus and placental membranes, complemented by bacteriology, serology, and real-time PCR analyses of different types of samples. For the specific diagnosis of C. burnetii as the causative agent of abortion, we provide guidelines based on expert opinions for the interpretation of laboratory test results in relation to their diagnostic value.

Plain Language Summary

Q fever, caused by the bacterium Coxiella burnetii, can be transmitted from animals to humans. It may cause reproductive issues in animals, such as cows, sheep, and goats, including miscarriages and weak offspring. The EU-funded Q-Net-Assess project aims to understand the genetic variations in C. burnetii and their impact on disease spread, severity, and control. Researchers in this project reviewed European methods for diagnosing miscarriages in these animals and created guidelines to identify causative agents, focusing on C. burnetii. The guidelines specify the necessary samples, diagnostic techniques, and interpretation of the results. The best diagnostic approach involves examining fetal and placental tissues using immunohistochemistry (IHC) supported by bacteriology, serology, and real-time PCR tests. For diagnosing C. burnetii, expert advice is provided to interpret the lab results.

Corresponding author

René van den Brom

0000-0001-9705-4184

r.vd.brom@gdanimalhealth.com

Grant information

This research was made possible by funding from ICRAD, an ERA-NET co-funded under European Union's Horizon 2020 research and innovation programme (https://ec.europa.eu/programmes/horizon2020), under Grant Agreement n°862605. UK partners received funding from the Biotechnology and Biological Sciences Research Council Grant reference: BB/X020142/1. French Partners received funding from the Agence Nationale de la Recherche (ANR) under Grant Agreement "ANR-22-ICRD-0001-06". Spanish Partners received funding from MICIU/AEI /10.13039/501100011033 under Project PCI2023-143391. Belgian partners received funding by the Federal Public Service of Health, Food Chain Safety and Environment contract RI 23/50. German partners received funding from the Federal Ministry of Food and Agriculture under project number 2823ERA30D within the framework of ERA-NETs ICRAD as part of “Improved molecular surveillance and assessment of host adaptation and virulence of Coxiella burnetii in Europe” (Q-Net-Assess). Royal GD received funding by the Dutch Ministry of Agriculture, Fisheries, Food Security and Nature.

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

© 2025 René van den Brom (Corresponding Author, Co-Author); Susan Neale (Co-Author); Elsa Jourdain (Co-Author); Anneleen Matthijs (Co-Author); Marcella Mori (Co-Author); Elodie Rousset (Co-Author); Katja Mertens-Scholz (Co-Author); Tom N. McNeilly (Co-Author); Ana Hurtado (Co-Author)

This work is licensed under a Creative Commons Attribution 4.0 International License.

First version published

March 31, 2025 (https://doi.org/10.12688/openreseurope.19270.1)

Latest version published

June 30, 2025 (https://doi.org/10.12688/openreseurope.19270.2)

Introduction

Q fever is a widespread zoonotic disease caused by the bacterium, Coxiella burnetii. Infections caused by C. burnetii are referred to as coxiellosis in animals and Q(uery) fever in humans (Plummer et al., 2018; WOAH, 2018). However, since the publication of case definition guidelines in the WOAH Terrestrial Code in 2024, the preferred terminology promoted by WOAH is 'Infection by Coxiella burnetii' (WOAH, 2024). Clinical symptoms in humans range from flu-like symptoms to persistent and potentially fatal infections, although asymptomatic infections and flu-like symptoms are more common than the latter (Angelakis & Raoult, 2010; Eldin et al., 2017; Maurin & Raoult, 1999). Transmission is usually airborne and results from inhalation of infectious dust particles or aerosols (Angelakis & Raoult, 2010; Clark & Soares Magalhães, 2018). Ruminant livestock, particularly sheep and goats, are the primary sources of human infections, although C. burnetii can infect a wide range of other animals, including wildlife, domestic birds, and pets (Angelakis & Raoult, 2010; Celina & Cerny, 2022; EFSA, 2010; Marrie et al., 1988; Maurin & Raoult, 1999; WOAH, 2018). It can also infect ticks, which may act as vectors and reservoirs, although their role in the epidemiology of the disease has likely been overestimated in some studies owing to diagnostic difficulties in distinguishing C. burnetii from Coxiella-like endosymbionts within tick populations (Duron et al., 2015; Jourdain et al., 2015; Körner et al., 2021; Yessinou et al., 2022).

In ruminants, C. burnetii can cause abortion, stillbirth, premature birth, and weak offspring (the ASPW complex). Coxiellosis may therefore lead to significant economic losses, particularly in small ruminants (goats and, to a lesser degree, sheep), in which abortion waves affecting all breeding females of a herd may occur (EFSA, 2010). In cattle, most infections are asymptomatic and abortions are sporadic. Disorders such as subfertility, endometritis/metritis, and retained fetal membranes (RFMs) have been associated with C. burnetii infection status in cattle, although direct evidence linking C. burnetii infection with these disorders is currently lacking (Agerholm, 2013). Furthermore, a recent systematic review concluded that although associations between C. burnetii infection status and RFMs and infertility/subfertility have been suggested, the association between endometritis/metritis in cattle and C. burnetii infection is far from clear (Gisbert et al., 2024).

Overall, the host range and infection outcome are highly variable. Although host factors may contribute to this outcome, our understanding of how C. burnetii genotype contributes to this variation is limited. Currently, the main genotyping methods used for C. burnetii generate limited genomic information and are difficult to standardize between laboratories. Whole genome sequencing (WGS) provides comprehensive genetic information and is easily standardized between laboratories and consequently, WGS has revolutionized the molecular epidemiology and surveillance of many zoonotic pathogens. However, due to difficulties in isolating the bacteria from field samples, few C. burnetii strains are currently available for WGS.

In 2023, a consortium consisting of partners from Belgium, France, Germany, Spain, the Netherlands, and the United Kingdom began with the EU-funded project Q-Net-Assess as part of the ICRAD (International Coordination of Research on Infectious Animal Diseases) initiative. The project aims to generate the most comprehensive understanding of C. burnetii genetic variation and to determine the implications of this genetic variation for zoonotic risk, livestock pathogenicity, and control of C. burnetii infection.

Work package (WP) 1 is aimed at collecting C. burnetii positive field samples from a range of hosts and environmental sources to obtain strains for isolation methods optimisation (WP2), WGS (WP3), as well as phenotypic and genotypic analyses (WP4). The collection included both animal and human samples and was built from archived and prospectively collected samples. Archived samples were obtained from the biobanks of human and animal origin samples from different consortium partners. Prospective animal sample collection mainly includes samples from cases of abortion in ruminants, as well as other types of samples from ruminant farms, such as bulk tank milk (BTM), environmental samples, and wildlife whenever available. Prospective sampling of C. burnetii associated with abortions in ruminants is being performed using coxiellosis surveillance systems already in place and under local mandatory monitoring programs in some of the participating countries. Laboratory procedures used at different laboratories for routine testing for concurrent abortifacients were shared and compared to ensure that they all comply with the minimum requirements for the investigation of C. burnetii and share equivalent criteria for case definition.

This manuscript compiled the information gathered from a review of the protocols used by different project partners and/or countries. It identifies the essential and complementary samples needed for an accurate diagnosis, describes the interpretation and validity of each type of sample and analytical techniques to detect C. burnetii as the causative agent of the abortion, and establishes the minimum requirements for C. burnetii investigation and the criteria for case definition.

Methods

At the commencement of the project, all partners involved were requested to submit the protocols applied in their institute/country to investigate abortions in ruminants. Partners included national reference laboratories for C. burnetii in animals (ANSES, APHA, FLI) and both animals and humans (Sciensano), as well as research organizations (APHA, INRAE, MRI, NEIKER, and Royal GD), all specializing in animal health, including coxiellosis. Some of these institutes perform (NEIKER, APHA, MRI) or even centralize the diagnostic process in their country (Royal GD), while others (ANSES, FLI, INRAE, Sciensano) do not routinely investigate abortions in ruminants and have reported the methods collected from networks of veterinary diagnostic laboratories in their respective countries. During the in-person kick-off meeting of the project (31 May 2023), concept protocols for abortion diagnostics were discussed. Different types of samples, diagnostic tools used, and agents investigated were inventoried using standardized templates, which were the drafts of the Table 2–Table 3 that are finally incorporated within this manuscript. Partners gathered this information during discussions with national institutes involved in abortion diagnostics. These protocols were completed by partners and discussed in four online project working group meetings (7 July 2023, 13 October 2023, 12 January 2024, 12 April 2024). After these meetings, a preliminary draft report was written and shared with partners, which was discussed during the annual internal in-person project meeting (30 May 2024). Based on the input from all partners, the final version of the report was written, subsequently discussed with all partners during two virtual working group meetings (12 July 2024 and 11 October 2024) and then approved by all partners before submission (December 2024). The protocols presented here are those in use as of February 2025.

Results

3.1 Investigation of ruminant abortion submissions

The analysis of the ruminant abortion protocols shared by the consortium partners showed that the pathogens investigated as the possible cause of abortion in ruminants, and the techniques used varied among different institutes and/or countries. The agents that were investigated included viruses (Schmallenberg virus, pestiviruses, bovine herpes virus), bacteria (C. burnetii, Chlamydia abortus, Listeria spp., Campylobacter spp., Salmonella spp., Leptospira spp., Mycoplasma spp. and other abortifacient bacteria which can be detected through aerobic and anaerobic culture), parasites (Neospora caninum and Toxoplasma gondii), and mycotic agents (Table 1). Testing for certain agents is restricted to specific ruminant species (e.g., T. gondii in sheep and goats; bovine herpesvirus only in cattle) or specific epidemiological contexts (i.e., presence of agents in a certain region) and therefore differs among countries, while other pathogens are the subject of EU and/or country-specific legislation (i.e., Brucella). The pathogens investigated and the diagnostic techniques used by each institute/country are presented in Table 2a –f. Protocols usually include a panel of pathogens that are routinely investigated, which vary according to the specific epidemiological context of each region, plus additional agents that are only investigated when specific clinical signs or pathological findings are observed or a diagnosis is not achieved using the standard protocol. Some countries follow standardized protocols that include the animals to be sampled, the type of sample(s), the tests to be performed, and the interpretation of the results for each disease (e.g. the Observatory of Causes of Abortions in Ruminants (OSCAR) protocol in France); others define (mandatory) tests supported by the government (e.g. the Belgian “Abortion Protocol” funded by the Federal Agency for the Safety of the Food Chain - FASFC). In other countries (e.g., Germany and Spain), there are no general guidelines for the diagnosis of abortions in ruminants. A combination of histopathology, bacterial culture, serology, and/or real-time PCR is used by all partners, using specific approaches that vary depending on the targeted etiological agent.

Table 1. Pathogens investigated as possible cause of abortion in ruminants in the different institutes and/or countries.

	Reporting partner	ANSES	APHA	FLI	NEIKER	Royal GD	Sciensano
	Scope	France¹	UK	Germany²	Spain³	The Netherlands	Belgium⁵
Pathogens	*Coxiella burnetii*	✓	✓	✓	✓	✓	✓
	Anaplasma sp.	✓	(✓)	NR	(✓)	NR	(✓)
	Brucella sp.	✓	✓	✓	✓	NR⁴	✓
	Campylobacter sp.	✓	✓	✓	(✓)	✓	✓
	*Campylobacter fetus* subsp. venerealis	✓	(✓)	✓	(✓)	NR⁴	NR
	*Chlamydia abortus*	✓	✓	✓	✓	✓	✓
	Leptospira sp.	✓	✓	✓	✓	✓	(✓)
	Mycoplasma sp.	NR	(✓)	(✓)	(✓)	✓	NR
	*Tritrichomonas foetus*	NR	(✓)	(✓)	(✓)	NR	NR
	Ureaplasma sp.	NR	(✓)	NR	(✓)	NR	(✓)
	*Other bacteria (e.g., Salmonella* sp., Listeria sp., Yersinia sp., Trueperella** pyogenes)	✓	✓	✓	✓	✓	✓
	*Neospora caninum*	✓	✓	✓	✓	✓	✓
	*Toxoplasma gondii*	✓	✓	(✓)	✓	✓	✓
	Bluetongue virus	(✓)	(✓)	(✓)	NR	(✓)	(✓)
	Bovine herpesvirus	NR	(✓)	✓	✓	✓	(✓)
	Pestivirus (Border disease virus-BD; Bovine viral diarrhoea virus-BVD)	✓	✓	✓	✓	✓	✓
	Schmallenberg virus	(✓)	(✓)	✓	(✓)	(✓)	(✓)
	*Mycoses (particularly Aspergillus* sp.)**	✓	✓	(✓)	(✓)	✓	✓

(√) In brackets, pathogens are not included in all cases in the standard abortion protocol; they are only investigated in particular circumstances based on specific clinical signs, epidemiological context, or post-mortem findings, and optionally, when a diagnosis is not achieved with the standard protocol. For example, Tritrichomonas foetus and Campylobacter fetus subsp. venerealis may be investigated in cases of infertility; Bluetongue virus and Schmallenberg virus if the fetus shows typical abnormalities, and the investigation of Anaplasma, Mycoplasma and Ureaplasma may be based on history and/or clinical findings. NR refers to those not routinely investigated, but tests can be performed if needed.

¹ The French laboratory network follows the OSCAR (Observatory of Causes of Abortions in Ruminants) protocol, a prototype scheme for differential diagnosis, that is voluntary. Pathogens not included (in brackets or denoted NR) were investigated in particular circumstances, as described above. The OSCAR protocol aims to identify infectious causes of abortion in ruminants (cattle, sheep, and goats) using harmonised diagnostic recommendations at the national scale. The network involves not only laboratories but also the entire chain of involved partners, starting from sampling on farms: the key stakeholders involved included GDS France, the National Animal Health Surveillance Platform (Plateforme ESA), farmers, practicing veterinarians, veterinary diagnostic laboratories, departmental veterinary services, and technical and research institutes. It is important to note that OSCAR's annual report does not provide nationwide surveillance data, as the system is still under development; therefore, the collected data are not representative of the entire French territory.

² There are no general guidelines for the diagnosis of abortions in Germany. Specific regulations exist for certain pathogens, e.g. Brucella. The panel described here is based on submission recommendations from seven state veterinarian diagnostic laboratories and five private diagnostic laboratories.

³ There are no general guidelines for the diagnosis of abortion in Spain. There are specific laws and regulations for certain pathogens (e.g., Brucella). The panel described here was based on the protocol used at NEIKER (Basque Country, Spain) for the investigation of abortion cases submitted for diagnosis.

⁴ The Netherlands is free of brucellosis, Campylobacter fetus subsp. venerealis and Tritrichomonas foetus; therefore, these pathogens are not included in the ruminant abortion investigation protocol. Brucellosis has been investigated in cattle by compulsory testing of aborted cattle. For small ruminants, sheep and goats from 1,475 farms are tested annually using the Rose Bengal Test.

⁵ In Belgium, the 'Abortion Protocol' is implemented for the diagnosis of ruminant abortions via the laboratories of ‘Dierengezondheidszorg Vlaanderen’ (DGZ) and ‘Association Régionale de Santé et d’Identification Animales’ (ARSIA). Beyond mandatory tests defined by disease-specific legislation (e.g., for brucellosis and coxiellosis), the basic protocol encompasses a comprehensive panel of other tests aimed at identifying the most common infectious causes of abortion in ruminants. See Table 2e for specific details. The panel of tests may change depending on the epidemiological situation, and the FASFC bears analysis costs. Next, DGZ/ARSIA offers additional tests for abortion diagnosis subject to a fee. The additional tests offered by the two laboratories may differ slightly.

Table 2a. Abortion diagnosis in France (reporting partners: ANSES and INRAE).

Pathogen / Test¹	Histopathology	Bacteriology (culture)	Serology	PCR or RT-PCR
*Coxiella burnetii*			S	VS; P; F; FSC
Anaplasma sp.			S	- A. marginale: B - A phagocytophilum: B; P; VS
Brucella sp.		VS (if serology positive)	S (mandatory)	VS (if serology positive)
Campylobacter fetus (subsp. fetus and venerealis - cattle)				ES
*Chlamydia abortus*			S	VS; P; F; FSC
Leptospira sp.			S	P; FSC; VS
Other bacteria (e.g. Salmonella sp., Listeria sp., Yersinia sp., Trueperella sp., Bacillus sp.)		Salmonella sp. & Listeria sp.: FSC; F (spleen, liver, brain); VS; P	Salmonella sp.: S (SAT)	Salmonella sp.: FSC (primarily); F (spleen, liver); VS
*Neospora caninum*	F (brain)		S	F (brain)
*Toxoplasma gondii*			S	F (brain, primarily; umbilical cord, possible); P)
Bluetongue virus*				B
Pestivirus (BD / BVD)			S	- BD: B (stunted or sick newborns); F (spleen primarily, brain, liver); P - BVD: F (spleen); P
Schmallenberg virus*	Congenital malformations			F (brain primarily, blood, spleen)
Mycoses (particularly Aspergillus)	If culture positive	P, FSC

B, maternal blood (in EDTA-tube); ES, endocervical swab; F, fetus; FSC, fetal stomach contents; P, placenta (taken from the uterus); S, maternal serum; VS, vaginal swabs. SAT, serum agglutination test.

¹ According to the OSCAR protocol except for some agents, indicated here by an asterisk, considered as abortifacients in specific contexts.

Table 2b. Abortion diagnosis in Great Britain, UK (reporting partner: APHA).

Pathogen / Test	Histopathology	Bacteriology		Serology (ancillary test)	PCR or RT-PCR
Pathogen / Test	Histopathology	Culture	MZN	Serology (ancillary test)	PCR or RT-PCR
*Coxiella burnetii*	P		P; FSC	B (ELISA)	P; FF
**Anaplasma sp.***	F				B
Brucella sp.		FSC; P	P; FSC	B (RBT, ELISA)
Campylobacter sp.		FSC
Campylobacter fetus subsp. *venerealis (cattle)**		FSC
*Chlamydia abortus*	P		P; FSC	B (ELISA)	P
Leptospira sp.	F			B (MAT, ELISA)	F (kidney)
**Mycoplasma sp.***	F	See PCR¹		B (M. bovis ELISA)	FSC (culture / DGGE / PCR)
Tritrichomonas foetus*		VF, SW²
**Ureaplasma sp.***	F	See PCR¹			FSC (culture / DGGE / PCR)
Other bacteria (e.g. Salmonella sp., Listeria sp., Yersinia sp., Trueperella sp., Bacillus sp.)	P, F	FSC
*Neospora caninum*	F			B (ELISA)	F (brain)
*Toxoplasma gondii*	P, F			B (LAT)	P (cotyledon)
Bluetongue virus*	F			B (ELISA)	B, F (spleen, brain)
Bovine herpesvirus (BHV-1)*	F			B (ELISA)	F (liver, lung)
Pestivirus (BD / BVD)	P, F			B (Antibody ELISA; antigen ELISA for BVD)	F (spleen or thymus)
Schmallenberg virus*	F			B (ELISA)	B; F (brain, spinal cord); FF
Mycoses (particularly Aspergillus sp.)	P, F	FSC (P)

B, maternal blood; FF, fetal fluids; F, fetus; FSC, fetal stomach contents; P, placenta; SW, sheath washings; VF, vaginal fluids. MZN, Modified Ziehl-Neelsen stain of smear; ELISA, enzyme-linked immunosorbent assay; LAT, Latex Agglutination Test; MAT, microscopic agglutination test; RBT, Rose Bengal test. DGGE, denaturing gradient gel electrophoresis.

* Pathogens not included in the standard abortion protocol.

¹ For Mycoplasma sp. (including Ureaplasma sp.), a combined test comprising culture, DGGE, and PCR.

² Tested by culture and microscopy

Great Britain is officially brucellosis free (Brucella abortus, B. melitensis, B. ovis). Ruminant abortion (including premature birth) submissions are screened for Brucella sp. as part of continued surveillance.

Table 2c. Abortion diagnosis in Germany¹ (reporting partner: FLI).

Pathogen / Test	Histopathology¹	Bacteriology (culture)	Serology	PCR or RT-PCR
*Coxiella burnetii*	F, P		S, Pm, M (ELISA)	F, FSC, LF, M, P, VS
Brucella spp.	F, P		S (RBT, CFT, ELISA)	F, P, VS
Campylobacter spp. (small ruminants)		F, LF, P, VS
Campylobacter fetus subsp. venerealis (cattle)		F, LF, P, PS, VS
*Chlamydia abortus*	F, P		S (ELISA)	F, LF, VS, P
Leptospira sp.	P		S (MAT)	F, VS
**Mycoplasma sp.***		M (M. bovis)	S, M (ELISA, M. bovis)
*Tritrichomonas foetus**		VS, PS		VS, PS
Other bacteria (e.g. Salmonella sp., Listeria *sp., Yersinia* sp.,, Trueperella sp., Bacillus sp.)**		F, FO, P, VS
*Neospora caninum*	F, P		S (ELISA)	F, LF
*Toxoplasma gondii**				F (brain), P
Bluetongue virus*			S (ELISA)	B, S, F
BHV-1 (cattle)	F, P		S (ELISA)	LF, VS
Pestivirus (BD / BVD)	F, P		S (ELISA Ab+Ag)	B, M, NS (BVD), EP (BVD)
Schmallenberg virus	F, P		S (ELISA)	B, F (brain), LF, VS
*Mycoses (particularly Aspergillus sp.)**		P, F

B: maternal blood; EP: ear punch sample; F: fetus (organs); FSC, fetal stomach contents; LF: lochial fluid; M: milk; NS: nose swab; P: placenta; Pm: plasma; PS: preputial smegma; S: maternal serum; VS: vaginal swabs.

CFT, complement fixation test; ELISA, enzyme-linked immunosorbent assay; MAT, microscopic agglutination test; RBT, Rose Bengal test.

* Pathogens not included in the standard abortion protocol.

¹ Histopathology is performed on request.

Table 2d. Abortion diagnosis in the Basque Country, Spain (reporting partner: NEIKER).

Pathogen / Test	Histopathology¹	Bacteriology		Serology	PCR or RT-PCR
Pathogen / Test	Histopathology¹	Culture²	MZN³	Serology	PCR or RT-PCR
*Coxiella burnetii*	F; P		P (F; VS)	S (ELISA)	P; F; VS
*Anaplasma phagocytophylum**					B
Brucella sp.	F; P	P; F; FSC; VS	P (F; VS)	S (RBT; CFT)
**Campylobacter sp. (small ruminants)***	F; P	P; F; FSC; VS
**Campylobacter fetus subsp. v enerealis***	F; P	P; F; FSC; VS; PS			VS; PS
*Chlamydia abortus*	F; P		P (F; VS)	S (ELISA)	P; VS; F
*Leptospira*	F; P			S; FF⁴ (MAT)	F (lung; kidney); U; M
*Mycoplasma**	F; P	P; VS
*Tritrichomonas foetus**	F; P	P; F; VS; PS			VS; PS
*Ureaplasma**	F; P	P; F; VS
*Other bacteria (e.g. Salmonella* sp.,** Listeria sp., Yersinia sp., Trueperella sp., Bacillus sp.)	F; P	P; F; FSC; VS
*Neospora caninum*	F; P			S (ELISA)	P, F
*Toxoplasma gondii*	F; P			S (ELISA)	P, F
Bovine herpesvirus (BHV-1)	F; P			S (ELISA)	P, F
Pestivirus (BD / BVD)	F; P			S; FF⁴ (ELISA)	P; F; B
Schmallenberg virus*	F; P			S (ELISA)	P; F; Me; B
Mycoses (Aspergillus)*	F; P	P

B: maternal blood, F: fetus, FF: fetal fluids, FSC, fetal stomach contents, M: milk, Me: meconium, P: placenta, PS: bull preputial smegma, S: maternal serum, U: urine, VS: vaginal swabs. MZN, Modified Ziehl-Neelsen stain of smear; CFT, Complement Fixation Test; ELISA, enzyme-linked immunosorbent assay; MAT, microscopic agglutination test; RBT, Rose Bengal test.

* Pathogens not included in the standard abortion protocol.

¹ Fetal organs taken for histopathology include the CNS (Pestivirus, Schmallenberg, Toxoplasma & Neospora), liver (Coxiella, BHV1), kidney (Leptospira); thyroid (Pestivirus), spleen (Pestivirus), lung (Coxiella, BHV1, Leptospira); lip and eyelid (Pestivirus), skeletal and cardiac muscle (protozoa), and skin (Mycoses).

² Fetus (F) for bacterial culture refers to liver.

³ For MZN the placenta is preferred; if not available, fetus (organ or stomach content) and/or vaginal swabs are analysed instead.

⁴ Serology of fetal fluids for immunocompetent fetuses.

Table 2e. Abortion diagnosis in The Netherlands (reporting partner: Royal GD).

Pathogen / Test	Histopathology¹	Immuno- histochemistry (IHC)¹	Bacteriology (culture)	Serology	PCR or RT-PCR
Coxiella burnetii²	F; P	F; P		S (ELISA)	F; P
Brucella sp.³				Mandatory sampling of maternal sera from aborted cattle (RBT)
Campylobacter sp.	F; P		FSC
*Chlamydia abortus*	F; P	F; P		S (ELISA)	F; P
Leptospira sp.	F; P	F; P		S (cattle)
*Mycoplasma*				S (M. bovis in cattle)
Other bacteria (e.g. Salmonella *sp., Listeria* sp., Yersinia sp.,** Trueperella sp., Bacillus sp.)	F; P		FSC	Salmonella in cattle: S (ELISA)
*Neospora caninum*	F; P			S (cattle)
*Toxoplasma gondii*	F; P	F; P			F; P
Bluetongue (BTV)*	F			Fetal serum (ELISA)	F (if the fetus shows typical abnormalities)
Bovine herpesvirus (BHV-1)	F; P	F		S (IgE ELISA)	F
Pestivirus (BD/BVD-small *ruminants; BVD-cattle)**	F			S (ELISA)	F (antigen ELISA)
**Schmallenbergvirus***	F			Fetal serum (ELISA)	F (if the fetus shows typical abnormalities)

F, fetus; P, placenta; S, maternal serum. ELISA, enzyme-linked immunosorbent assay; RBT, Rose Bengal test.

* Pathogens not included in the standard abortion protocol unless the fetus shows typical abnormalities fitting BTV or SBV.

¹ Fetal tissues taken for histopathology and IHC include: CNS; liver; lung cardiac muscle (protozoa).

² In sheep and goats, coxiellosis is a notifiable disease with an increased number of abortions notification criterion.

³ Brucella is not included in the ruminant abortion pathology protocol since The Netherlands is free. Brucellosis has been investigated in cattle by compulsory testing of aborted cattle. For small ruminants, sheep and goats from 1,475 farms are tested annually by serology (RBT).

More tests are not included in the table because samples are collected by the farmer: vaginal swabs of small ruminants for Chlamydia abortus PCR, deep throat swabs of aborted lambs/kids for PCR, and bacteriological examination for multiple causes of abortion.

Table 2f. Abortion diagnosis in Belgium (reporting partner: Sciensano-DGZ).

Pathogen / Test	Bacteriology		Serology	PCR	Other
Pathogen / Test	Culture	MZN	Serology	PCR	Other
*Coxiella burnetii*		P; F (SR¹)	S (ELISA – C², SR²)	P; F (C², SR¹)
*Anaplasma phagocytophilum**				P; F (C²)
Brucella spp.	P; F (C¹, SR¹ if MZN is positive)	P; F (SR¹)	S (SAW, MAT, ELISA – C¹, SR¹)
Campylobacter spp.	F (C², SR¹)
*Chlamydia abortus, Chlamydia* spp.		P; F (SR¹)	S (ELISA C. abortus - SR¹)	Chlamydia spp.: P; F (C², SR¹ if MZN-positive)
**Leptospira sp.***			S (L. Hardjo ELISA - C²)	P; F (C²)
**Ureaplasma sp.***				U. diversum: F (C²)
Other bacteria (e.g. Salmonella sp., Listeria sp., Yersinia sp., Trueperella sp., Bacillus sp.)	F (C², SR¹)
*Neospora caninum*			S (ELISA – C¹, SR²); F-blotting paper (ELISA – C², SR²)	F; P (C²)	histopathology on F (heart and brain) – C²
*Toxoplasma gondii*				F; P (SR¹)
Bluetongue virus*			S (C², SR²)	F – C², SR²
Bovine herpesvirus (BHV-4)*				F (C²)
Pestivirus (BD / BVD)					F (ear notch) antigen ELISA – C¹; S (if F tests positive) antigen ELISA – C¹
Schmallenberg virus*			S (C², SR²)	F-C²SR²

F, fetus; P, placenta; S, maternal serum. MZN, Modified Ziehl-Neelsen stain of smears. ELISA, enzyme-linked immunosorbent assay; MAT, microscopic agglutination test; SAW; Slow Agglutination of Wright.

* Pathogens not included in the standard abortion protocol.

¹ indicates that testing is part of the basic “Abortion Protocol” funded by FASFC; ² additional tests for abortion diagnosis offered by DGZ and/or ARSIA and subject to a fee. The conditions applied to each animal species are as follows: cattle (C), sheep, and goats (SR).

Other tests performed within the basic protocol funded by FASFC are necropsy of the fetus (cattle, sheep and goats) and general mycological examination of the fetus in sheep and goats. Other additional tests offered by DGZ and/or ARSIA and subject to a fee include histology, minerals, and vitamins (Se, I, Vit A, Vit E) on maternal sera, fetus (when suspected of Se or I deficiency), immunoglobulins, IgM, and serum amyloid A (SAA) in fetal thoracic fluid.

If the fetus or afterbirth cannot be recovered, a dry vaginal swab is collected from the dam.

Methods for the diagnosis of C. burnetii infection are presented in Table 3. C. burnetii PCR is conducted by all (6/6) partners in cases of abortion in ruminants based on several matrices, mainly the placenta, fetal tissues, and vaginal fluids from aborted females. Histopathology of the fetus and placenta is performed by four out of six (4/6) partners, although only Royal GD combines this with immunohistochemistry (IHC) in cases of placentitis as a diagnostic tool in (small) ruminants. Bacteriology for C. burnetii is mainly based on microscopic examination of smears (placenta, fetal stomach content, and/or vaginal discharge) stained using modified Ziehl-Nielsen, and it is applied by four out of six (4/6) partners. The presence of antibodies against C. burnetii in maternal serum is available by serology in six out of six (6/6) partners, although different commercial ELISA kits were used.

Table 3. Coxiella burnetii investigation in abortion submissions by the different partners.

Reporting Partner	Histopathology	Bacteriology¹	Serology	Real-time PCR
ANSES			S (ELISA)	VS; P; F; FSC²
APHA	P	P; FSC (MZN)	S³ (ELISA)	P; FF⁴
FLI		P; F; FSC; VS; M (culture)	S; Pm; M (ELISA)	P; F; FSC; VS; M
NEIKER	P; F⁵	P; F; VS (MZN)⁶	S (ELISA)	P; F; VS
Royal GD	P; F (including IHC⁷)		S (ELISA)	VS⁸; F; P (cattle)
Sciensano	F	P; F (MZN)	S (ELISA)	F⁹; P; VS; B

B: maternal blood; BTM: bulk tank milk; F: fetus; FF: fetal fluid; FSC, fetal stomach contents; M: milk; P: placenta; Pm: plasma; S: maternal serum; VS: vaginal swab. IHC, immunohistochemistry; MZN, Modified Ziehl-Neelsen stain of smear; ELISA, enzyme-linked immunosorbent assay.

¹ Bacteriology refers to MZN smears or culture isolation as stated.

² For PCR, vaginal swabs are the preferred samples (essentially because they are optimal for preventing zoonotic transmission to the operators during shipment, storage, and analysis); when the test is performed on the placenta (collected in the uterus), a pool of three cotyledons is used; the test on the aborted fetus targets the spleen, liver, or stomach contents. Semi-quantitative PCRs (relative to a “clinical threshold”) or quantitative PCRs are performed.

³ Serology as ancillary test.

⁴ PCRs are performed when C. burnetii is detected or suspected (on MZN smears) and for all statutorily reported positive farms.

⁵ Fetal organs analysed are primarily liver and lung.

⁶ If placenta is not available, fetal (organ or stomach content) and/or vaginal swabs are analysed instead.

⁷ Immunohistochemistry is performed in case of placentitis.

⁸ Official protocol Dutch Food and Consumer Safety Authority in case of an increased number of abortions on small ruminant farms.

⁹ In the fetus, the abomasum, abomasum content, spleen, and liver are the preferred target, but all organs can be used. PCR is compulsory for small ruminants (part of the “Abortion protocol” funded by FASFC).

3.2 Interpretation of results of abortion submissions in relation to Coxiella burnetii

The interpretation of C. burnetii results by different methods and in different sample matrices in relation to abortion in ruminants is presented in Table 4 and Figure 1. Samples that are mainly investigated by the consortium partners are placenta (including cotyledons), fetal samples (including fetal fluid), vaginal swabs, and maternal blood samples. The interpretation of results can be challenging and difficult to harmonize. Histopathology combined with IHC on placental membranes and cotyledons is considered the most reliable diagnostic technique for abortion diagnostics, but it is not as sensitive as real-time PCR.

Table 4. Interpretation of Coxiella burnetii results by different test methods and in different matrices/samples in relation to abortion in ruminants.

Technique	Sample	Prove of causality¹ (-, +/-, +, ++, +++)	Interpretation and conclusion
MZN Smears	Placenta/fetus	+	Unspecific result on its own; needs confirmation of presence of Cb by PCR.
Histopathology (HP) Typical pathological lesions and IHC positive	Placenta (fetus)²	+++	Lesions compatible with Cb are not pathognomonic; needs positive IHC or IHC positivity to prove Cb causality of the abortion.
Real-time PCR In general, Cb PCR on its own does not prove a relationship between the detection of the pathogen and the abortion, although both the type of sample investigated and the level of positivity influence the likelihood of causality. The higher the level of positivity, the more likely causality. Causality can be proven by HP in combination with immunohistochemistry.
	Fetus	++	Can be used to confirm the detection of Cb, in association with compatible histopathological findings. In the absence of compatible lesions, PCR positivity makes the relationship between pathogen and abortion more likely if other abortifacients have been ruled out.
	Placenta	++	Same interpretation as fetus but only if Cb PCR positivity level is high; low PCR positivity levels can be the result of sample contamination.
	Vaginal discharge	+	Indication for a putative relationship between abortion and Cb that needs further confirmation. Shedding of Cb can also occur after normal parturition.
	Bulk tank milk or individual milk sample (independently of Ct-value)	-	Detects shedding of Cb in a herd/flock or at the individual level. Does not prove a relationship between abortion and pathogen.
	Environmental sample dust/air/…) (independently of Ct-value)	-	Detects environmental contamination with Cb which may result in human exposure. Does not prove a relationship between abortion and pathogen. Tracing the most likely source of shedding may be advised depending on the context of the result.
Serology³	Maternal serum	-	Seropositivity shows previous contact with Cb, but no causality with abortion. The relationship between shedding/abortion and serology is poor.
Serology³	Milk	-

Abortion: Abortion, stillbirth, premature birth or weak offspring

MZN: modified Ziehl-Neelsen stained smears (unspecific method)

Cb: Coxiella burnetii

HP: Histopathology

IHC: immunohistochemistry (Cb specific antibodies are currently unavailable for every routine laboratory).

Ct: Cycle threshold

¹ Prove of causality based on expert opinion

² Placenta is more informative than the fetus

³ Positive serology can be a result of previous infection, vaccination or even cross-reaction

Figure 1. Interpretation of laboratory test results for Coxiella burnetii in relation to causality of abortion.

Scheme of proposed interpretation of laboratory test results in relation to their value for the diagnosis of Coxiella burnetii as the cause of the abortion (causality). For immunohistochemistry (IHC) and histopathology (HP), both the fetus and placenta can be used, and their diagnostic values are comparable. Ct, Cycle threshold; Pos, positive.

The most common PCR target used was the multicopy IS1111 element (used by 6/6 partners), as targeting a multicopy gene is anticipated to increase PCR sensitivity over a single-copy PCR target. PCR of the placenta, abomasal content of the fetus, and vaginal swabs was considered the most sensitive approach, particularly when IS1111 PCR was used. However, only when PCR is positive for placenta with relevant histopathological findings and/or fetal tissues, fetal fluid, or stomach contents from aborted fetuses test positive by PCR can C. burnetii be considered a potential cause of abortion. In other circumstances, causality can be suspected but not confirmed following a PCR-positive test. In these instances, other abortifacient agents need to be ruled out, and herd background and other laboratory results should be considered before reaching a diagnosis.

Serological results from maternal blood samples are even more difficult to relate to the cause of abortion, as not all infected animals seroconvert. In addition, positive serology (in non-vaccinated dams) provides information on previous exposure to C. burnetii, but not on the time of exposure; thus, causality cannot be established. Nevertheless, in some countries, serological sampling recommendations are used to investigate animals within a certain time period after they show reproductive disorders. Furthermore, it is currently not possible to differentiate between animals exposed to C. burnetii and those vaccinated against coxiellosis. In cases where C. burnetii is suspected to be the cause of abortion, additional sampling and/or testing should be conducted.

Discussion

Diagnosis of the cause of abortion in ruminants is complex, and identification of the specific etiology is not always achieved. Success depends on the samples available for laboratory analyses, the techniques used, and the additional clinical and epidemiological information available (e.g., serial abortions, history of affected animals, stressful conditions, possible co-infections, and other investigations that point towards an abortive infection). Here, a comparison of ruminant abortion protocols submitted by all participants showed some variation in the way abortion diagnostics were performed. This enabled the discussion of the minimum and best practice requirements for the detection of abortifacient pathogens, including C. burnetii.

To establish the best standardized protocol for ruminant abortion diagnosis, it is essential to consider that these protocols may need to evolve as scientific knowledge and available tools advance. In addition, cost-benefit considerations play a crucial role. Each additional test will improve the likelihood of detecting an agent but will also increase costs. The pathogens to be investigated should be adjusted according to the current epidemiological situation of animal diseases and the evidence available in each region. Diagnosis requires detection of the pathogen, usually by isolation and/or PCR, with histopathology aiding diagnosis by identifying compatible lesions, which, in some cases, may be pathognomonic (e.g., neosporosis). Currently, the protocols for abortion investigation in ruminants contain a tailored combination of histopathology, bacteriology, serology, and/or real-time PCR for abortifacient agents known to be present in a certain region. If routine laboratory techniques fail to reach a diagnosis when an infectious cause is probable, further efforts should concentrate on detecting less likely or new pathogens. An advantage of post-mortem examination is that it allows the detection of new pathogens and novel histopathological changes (Borel et al., 2014; Van den Brom et al., 2012; Van den Brom et al., 2021). In the future, molecular techniques capable of identifying multiple agents simultaneously would improve the broadness of abortion diagnostics in ruminants, although interpretation of the findings would need to be done with caution.

For the diagnosis of C. burnetii abortion, the most suitable samples are the placenta, fetus, and vaginal swabs from aborted animals, of which the latter have biosecurity advantages regarding transport and laboratory handling. The highest bacterial loads are found in placental membranes, with up to 10⁹bacteria per gram (Babudieri, 1959; Fournier et al., 1998). When placental samples are submitted, both intercotyledonary tissue and cotyledons should be included (Roest et al., 2012). C. burnetii replicates intensively within trophoblast cells of the placenta; these cells are present in the villi of cotyledons and in smaller numbers in the intercotyledonary tissue. It is important that the veterinarian collects appropriate samples from a fresh placenta and submits them according to laboratory requirements. The fetal samples of choice were the spleen, lung, liver, and abomasal contents (fetal stomach contents). Of these, the stomach content is the most practical sample to be collected. Furthermore, C. burnetii infection can progress in stages within fetal organs and may colonize the spleen before other organs. This suggests that multiple organs may need to be tested for fetal diagnosis, as non-detection of C. burnetii in one organ does not rule out its presence in other organs at any given time.

Histopathology of the fetus and placenta can identify lesions compatible with C. burnetii infection (Agerholm, 2013), but the placenta and many fetuses will have some degree of autolysis, which will compromise histopathological examination. As histopathological changes alone are not pathognomonic for C. burnetii, suspected lesions need to be further corroborated by additional staining of histological samples (e.g., IHC). Thus, histopathology combined with IHC of the placenta and fetus is a useful reference for establishing the role of C. burnetii as the cause of abortion in ruminants, although C. burnetii specific antibodies for IHC are not easily available in all routine laboratories.

PCR is a sensitive and specific method for detecting C. burnetii. PCR can also be used to confirm the presence of C. burnetii in samples with positive MZN smears (an unspecific result on its own), and when compatible histopathological lesions are observed in placenta/fetal samples, and IHC is not performed. Real-time PCR analysis of the placenta, fetal tissues, and vaginal swabs (from aborted animals within eight days post-abortion) was used by all partners to test for C. burnetii. However, causality of abortion cannot be proven based solely on the detection of C. burnetii DNA by PCR, although this is more likely when the estimated bacterial loads are high. The observation of low cycle threshold (Ct) values from real-time PCR is indicative of high bacterial loads, but caution must be taken since an “Inter-laboratory proficiency testing programme report” revealed significant differences in Ct values obtained by different laboratories (Rousset, 2021). The inclusion of a standard of known bacterial gene copy numbers in each PCR reaction could help control intra- and inter-laboratory variations in qPCR.

PCR tests targeting IS1111 are more sensitive than PCR tests designed to detect single-copy genes (EFSA, 2010; Jones et al., 2011). Because of this high sensitivity, PCR results with low bacterial load should be interpreted with care, as they can be the result of environmental contamination rather than shedding by the animal. In addition, because the IS1111 element is present in variable numbers in different C. burnetii strains, from seven to 110 copies according to Klee et al. (2006), the sensitivity of this method is biased towards samples that contain Coxiella isolates with higher IS1111 copy numbers. A French expert group has proposed a quantitative PCR threshold above which C. burnetii can be considered the likely cause of abortion, a guideline adopted at the European level (EFSA, 2010) that French partners are currently applying. Under this proposal, a causative relationship between abortion in ruminants and C. burnetii is considered highly probable when at least 10⁴ bacteria per gram of placenta or vaginal swab is detected. However, this threshold may be more appropriate for vaginal swabs than for placental tissue, where bacterial loads are often much higher because of the intense replication of C. burnetii in trophoblasts. Therefore, a higher threshold for placental samples might be considered to rule out contamination. The C. burnetii strain Nine Mile RSA493, which displays 20 copies of the IS1111 element, is considered as a reference for copy number estimation (EFSA, 2010). However, the use of a quantitative PCR threshold based on IS1111 amplification is debatable because the copy numbers of the target vary among C. burnetii strains. Accurate quantification of the bacterial load would require the use of a single-copy gene, such as com1 or icd, as the target; however, its use in routine analyses is hampered by a loss in sensitivity.

Serology detects antibodies against a specific agent following previous exposure of the sampled animal, but cannot discriminate between vaccinated and naturally exposed individuals. However, the presence of antibodies in individual maternal serum does not prove a relationship between abortion and a particular agent. In the case of C. burnetii, the relationship between positive serology and shedding of C. burnetii is poor at the individual level (Hogerwerf et al., 2014; Rousset et al., 2009). Several factors have contributed to this complexity. First, some animals may not generate an antibody response that is sufficiently strong to be detected by serological tests, falling below the detection threshold. In addition, some animals may exhibit delayed seroconversion, with antibody levels rising slowly and crossing the detection threshold one to two weeks after abortion. This delayed seroconversion can lead to false-negative results if samples are collected too early (Arricau-Bouvery et al., 2005; de Cremoux et al., 2012; Rousset et al., 2009). Consequently, the usefulness of serology for the diagnosis of abortions in individual animals is limited. However, at the herd level, seroprevalence levels of approximately 50% of the sampled animals may be indicative of active C. burnetii infection, requiring further testing for confirmation (EFSA, 2010). Thus, serology performed at the herd level provides valuable complementary information to the results of direct diagnostic methods (IHC and real-time PCR) performed on aborted individuals, but is insufficient to diagnose C. burnetii as the cause of abortion in individual cases.

In several countries, vaccination against Q fever with a non-DIVA (Differentiating Infected from Vaccinated Animals) compatible vaccine is applied, which should be considered when serology results are interpreted. In addition, the sensitivity and specificity of ready-to-use commercial kits should be considered because recent studies have revealed that significant variations exist between available ELISAs depending on the ruminant species (Lurier et al., 2021).

Although coxiellosis frequently results in clinical signs (the ASPW complex), particularly in small ruminants, shedding of C. burnetii also occurs in the absence of clinical signs after normal parturition (Álvarez-Alonso et al., 2020). Therefore, notification and investigation of ruminant abortions will not detect all possible sources of C. burnetii. Additional surveillance systems are required to detect shedding, subsequent environmental contamination, and human exposure. In the case of asymptomatic infection, detection of shedding in dairy flocks/herds is possible by the collection and testing of bulk tank milk (BTM) (Boarbi et al., 2014; Jansen et al., 2022; Van den Brom et al., 2015), a method routinely used in the laboratories of two out of six (2/6) partners. Environmental samples (dust and aerosols) can also be tested for C. burnetii by PCR, but positive results only provide information on the presence of the bacterium in the tested sample/material, and not on the source of environmental contamination with C. burnetii (De Bruin et al., 2013; Hurtado et al., 2017). In addition, PCR detection in the environment provides no information on viability or infectivity, which requires in vitro and/or in vivo infection models (Mori et al., 2013). However, it may prove to be a useful indicator, as it suggests that subsequent human and animal exposures are likely. Depending on the epidemiological context, such environmental indicators may highlight the importance of source tracing and additional measures to prevent further shedding (Hurtado et al., 2023). Additionally, human syndromic surveillance (where clusters of suspected Q fever cases are identified) in combination with geographic information system analyses can also help detect possible sources of C. burnetii shedding animals (van den Wijngaard et al., 2011).

Conclusion

In conclusion, it appears that abortion diagnosis protocols for ruminants are tailored by each of the partners, based on the infectious agents present in each region. Histopathology, including IHC, on fetal and placental membranes supplemented with bacteriology, serology, and real-time PCR, is currently used for the detection of infectious causes of abortion in ruminants. This is the broadest diagnostic approach to relate a detected agent to the pathological changes in the fetus and/or placenta. For the identification of C. burnetii as the causative agent of abortion, based on the consortium expert opinion, we provide some guidelines for the interpretation of laboratory test results in relation to their diagnostic value. PCR is the preferred diagnostic tool for the detection of C. burnetii shedding because it is very sensitive. However, because of this high sensitivity, real-time PCR results with low bacterial load should be interpreted with caution because they can be the result of environmental contamination rather than shedding by the animal.

Despite advances in diagnostic techniques, establishing a standardized protocol that balances sensitivity, specificity, practicality, and cost remains challenging. In the case of C. burnetii, future efforts should focus on harmonizing diagnostic protocols across regions and improving our understanding of its role in ruminant abortion to improve control and prevention strategies.

Ethics statement

Ethical approval and consent were not required.

Data and software availability

No additional data are associated with this article.

Author contribution

All authors were involved in the development of the project, conceptualization, methodology, visualization, and collection of information from their country or institution. RvdB and AH led this part of the project and prepared the initial draft of the manuscript, which was discussed by all the authors. All authors have read and approved the final version of the manuscript. TNM acted as a supervisor of this project.

Acknowledgements

The authors would like to thank all the people who contributed and shared information from their organization, which led to the construction of the protocols.

Faculty Opinions recommended

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Publication information

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Research Article

Published

March 31, 2025

DOI

https://doi.org/10.12688/openreseurope.19270.1

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June 30, 2025 (Version of Record 1.0)
March 31, 2025 (Published Manuscript Under Review 1.0)

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European Commission

Grant ID(s): 862605

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Keywords

ruminants, abortion, diagnostics, Coxiella burnetii

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René van den Brom

Small Ruminant Health Department, Royal GD, Deventer, 7400 AA, The Netherlands

Roles

Corresponding Author, Co-Author, Conceptualization, Data Curation, Formal Analysis, Funding Acquisition, Investigation, Methodology, Project Administration, Resources, Validation, Visualization, Writing – Original Draft Preparation, Writing – Review & Editing

0000-0001-9705-4184

r.vd.brom@gdanimalhealth.com

Susan Neale

Penrith Veterinary Investigation Centre, Animal and Plant Health Agency (APHA), Cumbria, CA11 9RR, UK

Roles