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System-Based Protection Method for High-Voltage Pulse Generator Switching Units in Biomass Electroporation

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  1. Alexander Golberg ,  
  2. Klimentiy Levkov
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Abstract

Background

High-voltage pulsed electric field (PEF) technology has emerged as a promising technique for enhancing cell membrane permeabilization for biotechnological and medical applications. Given the parametric instability and low resistivity of biomass when used as an electrical load, it is imperative for the switching unit to meet specific requirements in terms of load capacity and protection against overloads and short circuits.

Methods

We introduce a novel system for safeguarding against overload and short-circuit currents by optimizing existing hardware-software resources. Our approach hinges on using the energy storage capacitor (ESC) both for energy storage and as a current sensor. By monitoring voltage drop during discharge, we estimate current parameters. Rapid discharge signals a short circuit. The microcontroller calculates ESC discharge limits based on preset parameters within a defined timeframe. If this limit is surpassed, the system promptly halts current pulses and discontinues supply to the load, ensuring reliable protection for the switching unit.

Results

By employing a system-based protection approach, we are able to reduce the complexity of circuit design, thereby enabling a unified control strategy that remains consistent regardless of the number and type of transistors used. This approach eliminates the need for separate circuit solutions to address this issue.

Conclusions

By utilizing a system method to protect the switching elements from short-circuit currents, we established a unified system structure for the circuit protection that is independent of current surges at the pulse fronts, as well as the specific number and type of transistors and switching modules employed. This method not only enhances the efficiency and reliability of the technology but also simplifies the design process and ensures a unified approach to protection, regardless of the system's configuration.

Corresponding author
Alexander Golberg
0000-0001-8782-8879
Grant information

This project has received funding from the ERA-NET under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 723770) EuroNanoMed III MATISSE project. The authors also acknowledge support form TAU Zimin Center for technologies for better life and Spark Tel Aviv.

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright
© 2023 Alexander Golberg (Corresponding Author, Co-Author); Klimentiy Levkov (Co-Author) Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Authors & roles

Alexander Golberg

Department of Environmental Studies, Porter School of Environment and Earth Sciences, Tel Aviv University, Tel Aviv, Israel

Roles
Corresponding Author, Co-Author, Conceptualization, Formal Analysis, Funding Acquisition, Investigation, Project Administration, Resources, Writing – Original Draft Preparation, Writing – Review & Editing
0000-0001-8782-8879

Klimentiy Levkov

Department of Environmental Studies, Porter School of Environment and Earth Sciences, Tel Aviv University, Tel Aviv, Israel

Roles
Co-Author, Conceptualization, Formal Analysis, Investigation, Methodology, Resources, Software, Validation, Visualization, Writing – Original Draft Preparation, Writing – Review & Editing

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